ABSTRACT
BACKGROUND: Siglec9 has been identified as an immune checkpoint molecule on tumor-associated macrophages (TAMs). Nevertheless, the expression profile and clinical significance of Siglec9 + TAMs in colon cancer (CC) are still not fully understood. METHODS: Two clinical cohorts from distinct medical centers were retrospectively enrolled. Immunohistochemistry and immunofluorescence were conducted to evaluate the infiltration of immune cells. Single-cell RNA sequencing and flow cytometry were utilized to identify the impact of Siglec9 + TAMs on the tumor immune environment, which was subsequently validated through bioinformatics analysis of the TCGA database. Prognosis and the benefit of adjuvant chemotherapy (ACT) were also evaluated using Cox regression analysis and the Kaplan-Meier method. RESULTS: High infiltration of Siglec9 + TAMs was associated with worse prognosis and better benefit from 6-month ACT. Siglec9 + TAMs contributed to immunoevasion by promoting the infiltration of immunosuppressive cells and the dysfunction process of CD8 + T cells. Additionally, high infiltration of Siglec9 + TAMs was associated with the mesenchymal-featured subtype and overexpression of the VEGF signaling pathway, which was validated by the strongest communication between Siglec9 + TAMs and vascular endothelial cells. CONCLUSIONS: Siglec9 + TAMs may serve as a biomarker for prognosis and response to ACT in CC. Furthermore, the immunoevasive contexture and angiogenesis stimulated by Siglec9 + TAMs suggest potential treatment combinations for CC patients.
Subject(s)
Antigens, CD , Colonic Neoplasms , Sialic Acid Binding Immunoglobulin-like Lectins , Tumor-Associated Macrophages , Humans , Colonic Neoplasms/diagnosis , Colonic Neoplasms/pathology , Endothelial Cells , Prognosis , Retrospective Studies , Tumor Microenvironment , Tumor-Associated Macrophages/immunology , Antigens, CD/metabolism , Sialic Acid Binding Immunoglobulin-like Lectins/metabolism , Male , Female , Adult , Middle AgedABSTRACT
This paper presents an investigation into the ultimate and serviceability behavior of concrete beams strengthened in flexure with basalt-textile-reinforced polymer mortar (BTRM). The bond performance at the interface between the BTRM and concrete was studied by performing single shear tests, and the effectiveness of using an adhesion promoter and impregnated resin for bond enhancement was explored. The results suggested that using an adhesion promoter and impregnated resin can improve the interfacial stress transfer and ensure the tensile failure of the basalt textile in BTRM. Afterward, four-point bending tests were conducted to study the flexural performance of strengthened beams. It was found that the flexural strength of strengthened beams increased with the amount of textile, and the strength increase was more prominent for the strengthened beams with end anchorages. The increase in the failure force was up to 37% for the beam strengthened with five layers of the textile and an end anchorage. The calculated flexural strength exhibited a percentage error of no more than 7% compared to the test results. In addition, the Bischoff-I Equation can closely estimate the effective moment of inertia and provide an accurate prediction of deflection for strengthened beams.
